Comparison of effect of Zataria multiflora and Rosemarinus officinalis extracts on quality of minced frozen silver carp

The aim of the this study was to have a comparison between Zataria multiflora and Rosemarinus officinalis extracts on quality of minced frozen silver carp preserved in cold storage temperature -18°C for six months. In this research, one control and three treatments of minced silver carp were prepared as Tr1 (+ Zataria), Tr2 (+Rosemary) and Tr3 (mixed with Rosemary and Zataria) all in normal packaging. After quick freezing of samples in the spiral freezer they were preserved in cold storage temperature -18°C for six months. In this period, the changes of Peroxide Value, Total Volatile Nitrogen, Tio Barbituric Acid and Free Fatty Acids in all treatments were measured based on a predetermined schedule. The results of this investigation showed a significant effect of anti-oxidant in all treatments (p<.05) and it was concluded that Rosemary containing anti-oxidants maintains the best quality after six months of cold storage and corruption indices are not exceeded. Analysis of Peroxide Value, Total Volatile Nitrogen, Tio Barbituric Acid and Free Fatty Acids in different treatments during six months of reservation in freezing under the temperature of -18°C showed that the obtained results to the end of the reservation period for all treatments has increased and treatment containing Rosemary, with Peroxide Value 6.83±0.35meq.1000g^-1, Tio Barbituric Acid 2.1±0.1mgMAL.kg^-1 and Total Volatile Nitrogen with 19.16±0.28mg.100g ^-1 had the best quality of preservation compared with other treatments during the preservation period. Therefore, based on these results and statistical analyses, the effect of anti-oxidant and time in all treatments is significant (p<.05) and the treatments containing Rosemary had the best quality preservation status in -18°C and did not surpass perishing criteria during this period

The enrichment influences of Artemia urmiana with vitamin E and unsaturated fatty acids on growth, survival and salinity stress resistance in beluga (Huso huso) larvae

VWe assessed the effects of Aremia urmiana nauplii enriched with vitamin E (α -tochopherol acetate, MERCK, Germany) and unsaturated fatty acids (EPA 18% and DHA 12%) on growth, survival and stress resistance of Beluga (Huso huso) larvae during six weeks. Beluga larvae (mean body weight 69.8c6.2 mg) at the first feeding were fed in four treatments and three replications using A. urmiana enriched with unsaturated fatty acids (HUFA) containing 20% and 50% vitamin E (El and E2 treatments, respectively) and HUFA without vitamin E (HUFA group). The control group was fed on non-enriched Artemia. All treatments were fed with non-enriched Artemia for 5 days after the first feeding and then fed with enriched Artemia for 7 days. After period of enrichment, the larvae were fed with daphnia from 13 ^(th) to 40 ^(th) day. The Beluga larvae were then exposed to salinity stress (12 g/l) for two days after the 40 days feeding trial. Blood samples were obtained at the end of the first and second days in order to evaluate hematocrite index. Growth, survival and stress resistance were compared at the end of experimental period. Growth factors showed significant difference between the groups. The highest and the lowest growth were observed in El and control treatments, respectively (P<0.05). Larvae resistance to salinity stress was comparable between the enriched and control groups (P< 0.05). The results indicated that the enrichment of Artemia with essential fatty acids and vitamin E can improve some growth and stress tolerance factors in beluga sturgeon (H. huso) larvae

Study on effect of Rosmarinus officinalis and Zataria multiflora extracts on the stability of fatty acids in frozen silver carp minced

In this study, the effect of Rosemary and Zataria extracts on three different treatments in frozen Silver carp minced were studied in normal packaging. Therefore, a control and three treatments were defined: Treatment 1 - Control: frozen meat packaged in conventional Treatment 2: Frozen Silver carp minced + Zataria 300mg/kg in normal packaging Treatment 3: Frozen Silver carp minced + Rosemary 200mg/kg in normal packaging Treatment 4: Frozen Silver carp minced + Rosemary compound (100mg/kg) and Zataria (100mg/kg) in normal packaging After quick freezing of samples in the spiral freezer by individual quick freezing method, to maintain the cold temperature (-18)°C were transferred. For six month, Sampling and measurements to determine the fatty acid profile of the zero phase beginning in the first month and then every ten days, and 15 days in the second month of the third month after the monthly test. Identifying, defining and measuring the fatty acid profile by gas chromatography was performed. In this study, levels of both saturated and unsaturated fatty acids in three experimental and one control were identified as follows: A: SFA: (Saturated Fatty Acid): Meristic C14: 0/ Palmitic C16: 0 /Hepta decaenoic C17: 0 / Stearic C18: 0 / Arashidic C20: 0 B: (MUFA Mono Unsaturated Fatty Acid): Palmitoleic C16: 1-W7 / Oleic C18: 1-W9 /Gadoleic C20: 1 - W9 C: (PUFA Poly Unsaturated Fatty Acid): Linoleic C18: 2-W6 /α-Linolenic C18:3-W3 D: (HUFA High Unsaturated Fatty Acid): Arachidonic C20: 4 - W6 Eicosa panta enoic acid C20: 5- EPA/W3 Docosa hexa enoic acid C22: 6-DHA/W3. Results of this study was to determine keep frozen fish meat containing extracts of Zataria and Rosemary in freezing conditions, stability of different types of fatty acids, mono unsaturated fatty acids, poly unsaturated fatty acids, high unsaturated fatty acids, So that none of the fatty acids measured, were not observed increase or decrease Changes over time while maintaining the oxidation of fatty acids is minimized. The results obtained from the profile of fatty acids and their related indices and statistical tests show the treatment contains Rosemary extract show greater stability than thyme during storage (- 18)°C. According to studies, Frozen minced fish meat treated with extracts of Rosemary, was used until the end of the storage period

Quality assessment of fish burgers from deep flounder (Pseudorhombus elevatus) and brushtooth lizardfish (Saurida undosquamis) during storage at -18ºC

Microbiological, chemical and sensory changes of fish burgers prepared from deep flounder (Pseudorhombus elevatus) and brushtooth lizardfish (Saurida undosquamis) were determined during storage at -18ºC for 5 months. Microbiological counts were including total plate count (TPC), total coliform (TC), Staphylococcus aureus, Psychotropic and Escherichia coli decreased throughout the frozen storage . Reduction of microbial load in brushtooth lizardfish was higher than that in deep flounder, except for Staphylococcus aureus counts that was almost equal in both groups. There was a significant increase in pH value in both groups (P<0.05) in first and second months of storage only. Moisture content increased in both groups at the end of 5th month, with increase of moisture in deep flounder fish burgers being higher than that in brushtooth lizardfish burgers. TVB-N values in both groups increased significantly (P<0.05 and P<0.008 for deep flounder and brushtooth lizardfish burgers, respectively) at the end of the second month, however, there was a decrease or no significant change afterward. TBA value of deep flounder fish burgers had a significant decrease (P<0.05) as storage time continued, however, it increased significantly in brushtooth lizardfish burgers at the end of second month (P<0.006) following by a decrease at the end of storage period. Peroxide value (PV) in both groups increased significantly (P<0.05 and P<0.002 in deep flounder and brushtooth lizardfish burgers, respectively) during storage time but a significant decrease was observed at the end of third and fourth months (P<0.005 and P<0.001 in deep flounder and brushtooth lizardfish burgers, respectively). Sensory parameters (color, texture, taste and general acceptability) for two groups decreased significantly (P<0.003 for all parameters in 2 groups) during storage with deep flounder fish burgers receiving higher scores than brushtooth lizardfish burgers at the beginning and end of the storage period

Hepatitis B/C virus co-infection in Iran: A seroepidemiological study

Background/aims: As hepatitis B and C virus have the same transmission routes, dual infection may occur. The aim of this study was to determine the seroprevalence of HCV in HBsAg-positive subjects. Methods: 139 HBsAg-positive subjects were enrolled in the study. Serum samples were tested using ELISA method for anti-HCV antibodies. Chi-square and Fisher's exact tests were used to compare the proportions. Results: There were 68 (48.9%) males and 71 (51.1%) females. The mean age was 41.89±11.30 years. One case was excluded because of inadequate blood sampling. Anti-HCV antibody was positive in 17 (12.3%) of the 138 remaining subjects. Seropositivity of HCV was similar between female and male patients (p=0.69). Conclusion: The seroprevalence of co-infection with hepatitis B virus and hepatitis C virus in our study was higher than such reports from some countries (Italy), but was in line with worldwide prevalence (>10%)

Bilateral maxillary, sphenoid sinuses and lumbosacral spinal cord extramedullary relapse of CML following allogeneic stem cell transplant

Isolated extramedullary relapse of chronic myelogenous leukemia (CML) after allogeneic stem cell transplant is rare. There is a case report of a child who developed a granulocytic sarcoma of the maxillary and sphenoid sinuses and lumbosacral spinal cord mass 18 months after allogeneic bone marrow transplant for CML. He was presented with per orbital edema and neurological deficit of lower extremities and a mass lesion was found on spinal cord imaging. No evidence of hematologic relapse was identified at that time by bone marrow histology or cytogenetic. The patient died 1 month later with a picture of pneumonia, left ventricular dysfunction and a cardiopulmonary arrest on a presumed underlying sepsis with infectious etiology. Granulocytic sarcoma should be considered in the differential diagnosis of mass lesions presenting after allogeneic bone marrow transplantation for CML, even if there is no evidence of bone marrow involvement. © 2016, Tehran University of Medical Sciences (TUMS). All Rights Reserved

Gastrointestinal manifestations and dynamics of liver enzymes in children and adolescents with COVID-19 infection: A systematic review and meta-analysis

Objectives: To improve the knowledge on Coronavirus Disease (COVID-19) infection, we aimed to study the prevalence of gastrointestinal symptoms and the dynamics of liver enzymes in children infected with COVID-19. Methods: We performed a systematic search of all the articles published up to May 2020 in the following databases: PubMed-MEDLINE, Scopus, and Cochrane. We chose the fixed-or random-effect model for analysis based on the I2 statistic. The included data were analyzed to identify the prevalence of gastrointestinal symptoms (diarrhea, vomiting or nausea) and to identify the dynamics of liver enzymes in children and adolescents diagnosed with COVID-19. Results: We detected an overall prevalence of all gastrointestinal symptoms of 26 (95 CI: 0.18-0.35). The pooled prevalence of diarrhea and nausea/vomiting was 12 (95 CI: 0.08-0.16) with no heterogeneity (P = 0.19; I2 = 23.53) and 11 (95 CI: 0.05-0.17), respectively. The pooled prevalence of elevated ALT and AST and LDH was 12 (95 CI: 0.07-0.17), 14 (95 CI: 0.10-0.18) and 33 (95 CI: 0.12-0.54), respectively among the included studies. Conclusions: According to our systematic review and meta-analysis, 26 of the children and adolescents diagnosed with COVID-19 present gastrointestinal symptoms. This paper has provided a comprehensive overview of the prevalence of digestive symptoms of COVID-19 and of the liver enzymes dynamics in children and adolescents. © 2020, Author(s)

Effects of Chlamydia trachomatis infection on sperm chromatin condensation and DNA integrity

The present study was performed to investigate the relation of Chlamydia trachomatis infection to sperm chromatin/DNA integrity in a population of infertile men (male partner of infertile couples) from Iran. Blood, semen and first-void urine samples were obtained from 250 infertile men. Data were analysed with regard to the results of (i) serological analysis for specific antibodies to C. trachomatis in serum; (ii) the presence of C. trachomatis and DNA in first-void urine; and (iii) in the semen sample of the male partner, in addition to sperm analysis, four different tests (aniline blue, chromomycin A3, acridine orange and TUNEL) were used to detect sperm chromatin and DNA abnormalities. The main conclusions of the results were: (i) no evidence of C. trachomatis infection in semen samples was found; (ii) sperm DNA fragmentation and chromatin studies were not correlated with C. trachomatis diagnosis; (iii) the percentage of DNA fragmentation is positively correlated with the percentage of immotile sperm but negatively with semen volume, normal morphology; and (iv) in sperm chromatin evaluations, only the percentage of chromatin protamination was related to male age

HBV/HCV co-infection in Iran: A seroepidemiological based study

The aim of this study was to determine the seroprevalence of HCV in HBsAg positive subjects. In a population based single stage cluster sampling in Golestan province of Iran, 139 HBsAg positive subjects were detected and enrolled in the study. Serum samples were tested using ELISA method for anti-HCV antibodies. Chi-square and Fishers exact tests were used to compare the proportions. There were 68(48.9%) male and 71(51.1 %) female. The mean age was 41.89±11.30 years (25-64 years). One case was excluded because of inadequate blood sampling. Anti-HCV antibody was positive in 17(12.3%) of 138 remaining subjects. Seropositivity of HCV in female was higher than male (14.1% versus 10.4%, respectively) but the difference was not statistically significant (p = 0.52). No significant relationship was seen between Anti-HCV seroprevalence and demographic factors such as age, place of residence and marital status. The seroprevalence of co-infection with HBV and HCV in our study was higher than such reports from some countries (Italy); but it was in line with worldwide prevalence (>10%). A careful longitudinal evaluation of the viremia levels of both HBV/HCV viruses is essential for making a correct diagnosis and tailoring the appropriate therapeutic schedule in co-infected patients. © 2006 Asian Network for Scientific Information